To be safe, keep file names at 27 or fewer characters (to allow for the ".scf", ".ab1" etc.
#Sequencher uga windows#
Keep in mind that Windows may not show you the entire file name - the extension may be hidden, depending on the Explorer settings and the extension. (This problem may be related to the maximum length of file names on the Classic MacOS). You can easily reproduce (or eliminate) this problem by renaming files before the Phred analysis.In our tests, the same files were imported without problems when the name (with extension) was 31 characters or less, but not if the names were 32 characters or longer. Sometimes, just one or two of the imported sequences seem to have qualities, which may or may not make sense, while other sequences do not have qualities at all.
![sequencher uga sequencher uga](https://geauganews.com/wp-content/uploads/2021/03/gEAUGA-TEACHER-WORKSHOP-SCHOLARSHIPS-1200-X-630.png)
Some of the most common reasons for these problems are: The sequences have wrong file names (like seq.fasta #1) and no qualities.
![sequencher uga sequencher uga](https://scga.org/pdfs/volunteers/16106/han3.jpg)
![sequencher uga sequencher uga](https://www.geaugamapleleaf.com/wp-content/uploads/2020/02/Geauga-SWCD-squashed.jpg)
The qualities shown in Sequencher do not make sense.You cannot open the chromatograms after importing.Importing does not import all the sequences in the fasta file.Sequencher does not show the qualities, or shows qualities only for some of the imported sequences.Sequencher gives an obscure error message when trying to import.Sequencher is a leading tool for contig editing and assembly developed by Gene Codes Corporation.Ī number of things can go wrong when trying to import qualities from a Phred-generated. For general instructions on importing Phred qualities into Sequencher, click here. This page describes some problems you may encounter when trying to import files with Phred qualities into Sequencher version 4. Sequencher Troubleshooting Sequencher Troubleshooting